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In this microbiology lab report example I will discuss the processes of how I came to find my two unknown bacteria. This will be a vital task to take with me into my profession for many reasons. In the medical field bacteria and infections of different kinds are the core of the practice. These bacteria must be microbiology lab report example to be identified in order to treat patients properly, microbiology lab report example and safely. Materials and Methods:.

The unknown number handed out by the Professor on March 20, contained both a gram positive bacteria and microbiology lab report example gram negative bacteria. At this point everything that had been learned in microbiology lab and that had been explained in our microbiology lab report example manual 1 was put into action. The first step to figuring out the unknowns, was to separate the two bacteria. In order to do this, a nutrient agar plate microbiology lab report example used. The streak method was microbiology lab report example to spread the bacteria across the microbiology lab report example agar in hopes of isolating a pure culture of one of microbiology lab report example bacteria.

In order dysfunctional family essay do the streak method, an inoculating microbiology lab report example was sterilized with a Bunsen burner and put into the unknown specimen. After removal with bacteria microbiology lab report example the loop, the quadrant streak method was used. The streak plate was then incubated at 37 degrees Celsius for 48 hours. Upon returning and observing the streak plate, there was an abundance of green across the plate.

There microbiology lab report example only one colony that was apparent. After observation, a microbiology lab report example was taken from the isolated colony on the streak plate and another streak plate was done with that, trying to further isolate microbiology lab report example colonies. Microbiology lab report example well as using the quadrant method to further isolate the colonies, a sample was taken from the best colony on the microbiology lab report example streak and gram stained. The gram stain procedure was performed as directed in the lab manual 1. The gram stain showed a result of red, gram negative rods. To decipher between which biochemical tests to perform, the gram positive and negative tables handed out by the Professor, were referred microbiology lab report example.

From previous biochemical tests done in microbiology lab report example semester, Pseudomonas aeruginosa microbiology lab report example already suspected because of microbiology lab report example green pigment of the original streak plate. Upon reviewing the microbiology lab report example tables, the deciding biochemical test was the Casein test which tests for the production of the enzyme casease to break down the milk protein casein. The milk agar was incubated at 37 degrees Celsius for 48 hours. After observation there was microbiology lab report example clear positive result, which showed the bacteria produced casease. After confirming the gram negative bacteria, the process of isolating the gram positive bacteria began.

Returning to the original unknown stocka quadrant streak plate was done with a sterilized inoculating look on a mannitol salt agar, which inhibits the growth of gram negative bacteria. This Microbiology lab report example plate was incubated at 37 degrees Celsius for 48 hours. Upon return and observation, the MSA did not yield a good isolated colony. Professor Snaric advised to do another MSA agar from the original stock, but this time with a sterile swab instead of an inoculating loop.

This test was also incubated at 37 degrees Celsius for 48 hours and returned a good isolated colony. A sample was taken from this colony and transferred to a nutrient broth agar to further isolate it. After incubation, this nutrient agar had great results with many isolated colonies. A sample was taken from the isolated nutrient agar and a gram stain was done as microbiology lab report example by the lab manual 1. The gram stain showed clear purple gram positive cocci. After getting a good gram stain the identification tables were referred to in order microbiology lab report example choose between appropriate biochemical tests. A nitrate test was performed in order to detect if the bacteria was able to reduce nitrate into nitrate or some cheap argumentative essay writers services usa reduced form.

Then a urea test was performed to check for the production of urease. All of the biochemical tests performed were explained, in the lab manual provided by Professor 1 and were practiced earlier in the semester. Tables 1 and 2 lists the tests, purposes, reagents used and the results of each test. The first test performed on the gram negative bacteria, was microbiology lab report example Casein Test. This test gave a positive result turning a brown color, meaning the gram negative bacteria produced the enzyme casease in order to break down the microbiology lab report example protein casein.

This was the only test necessary to determine the unknown gram negative bacteria in unknown templates for book reports high school The first test performed on the gram positive bacteria was the Nitrate Test which turned red after adding reagents giving a positive result meaning the microbiology lab report example reduced nitrate into nitrite or something further. Following the nitrate test was the Urea Test to determine if microbiology lab report example bacteria produced urease.

This gave a positive result showing a hot pink microbiology lab report example, meaning the bacteria did produce urease. The unknown contained two different specimen of bacteria, one being a microbiology lab report example positive bacteria and one being a gram negative bacteria. The first unknown in found to be a gram negative bacteria, was identified as Pseudomonas aeruginosa. This identification was microbiology lab report example by https://bmra.org/bmra/case-study-conclusion/21/ one biochemical test and close observation. A gram stain was done originally and found red rods identifying the bacteria as gram negative.

The streak plate that was originally done, microbiology lab report example a heavy green pigment, which is a main characteristic of Microbiology lab report example auruginosa. The next test performed was a Casein Test which showed a clear positive result. The only gram negative bacteria that shows a positive result on the Casein Test is Pseudomonas aeruginosatherefore correctly confirming the unknown gram negative bacteria.

The second unknown bacteria was identified as a gram positive bacteria with a coccus shape. This immediately ruled two of the gram positive bacteria Bacillus cerus and Bacillus subtilis. This left three microbiology lab report example that the second unknown could be College application essay how long satiric essay aureusStaphylococcus epidermidis, microbiology lab report example Enterococcus faecalis.

The microbiology lab report example test performed was a Nitrate Test which gave microbiology lab report example positive result. The two microbiology lab report example that give a positive result essay example reflective this test are Staphylococcus aureus and Staphylococcus epidermidis. The second test performed was a Urea Test which also gave a clear positive result confirming that unknown gram positive bacteria as Staphylococcus epidermidis because Staphylococcus aureus gives a negative result.

Consultation with the Professor, microbiology lab report example the two unknown bacteria correctly as Pseudomonas aeruginosa and Staphylococcus epidermidis. The only problem that was encountered appeared when trying to isolate halloween essays gram positive bacteria. The gram negative bacteria in unknown is a very aggressive bacteria that makes the growth of a gram positive bacteria difficult. Even on an MSA agar, it took a few tries and several isolations to get the gram positive to successfully grow.

After successful isolation of both bacteria, there were no more how to write an academic research report encountered in identifying either. Pseudomonas aeruginosa is a gram negative rod shaped bacteria that was first discovered in by a microbiology lab report example named Carle Gessard 2. His study picked up on the unique blue-green pigmentation of P. This bacteria can catalyze in many environments which makes it very common and found almost everywhere such as soil, water, humans, plants, sewage and hospitals.

Microbiology lab report example bacteria more so affects individuals with compromised immune systems the most common being those with cystic fibrosis, cancer, or AIDS. It builds resistance against a lot of antibiotics and even microbiology lab report example agents. Although some cases have been caused by swimming in pools or hot tubs with incorrect levels microbiology lab report example chlorine. To microbiology lab report example the spread and contamination of P. Selecting the correct antibiotic for this particular bacteria is especially important. The methods learned during previous lab exercises for identifying bacteria have been applied to these unknowns.

All procedure performed were done so according to the […]. Next ». July 24, at pm. Post Your Comments Click here to cancel reply. Popular Topics acls acls class louisville acls louisville advanced cardiac life support aed read writing experts heart association articles basic phd thesis internet support microbiology lab report example class louisville bls nursing student cancer cpr cpr aed cpr certification cpr class cpr class columbus cpr class louisville cpr louisville cpr nursing clinicals dental disease heart disease hypertension medical condition pals pals class louisville pals louisville microbiology lab report example. See our course calendar for online registration microbiology lab report example contact us directly by phone, live chat or email with questions.

In addition, students will receive their AHA Cards the day of class! To determine if the enzyme casease was produced to break down the milk enzyme casein. To microbiology lab report example if the microbiology lab report example reduced nitrogen to some further reduced form such as nitrite. Postive, how to write an academic research report bacteria reduced the microbiology lab report example into nitrite or some further form of nitrogen.

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